Cancer immunophenotyping by seven-colour multispectral imaging without tyramide signal amplification

Tyramide Signal Amplification Strategies for Fluorescence Labeling

Objective Colour from Multispectral Imaging

The light reflected from a surface depends on the scene geometry, the incident illumination and the surface material. One of the properties of the material is its albedo ρ(λ) and its variation with respect to wavelength. The albedo of a surface is purely a physical property. Our perception of albedo is commonly referred to as colour. This paper presents a novel methodology for extracting the al...

High Resolution Fluorescent In Situ Hybridization in Drosophila Embryos and Tissues Using Tyramide Signal Amplification

In our efforts to determine the patterns of expression and subcellular localization of Drosophila RNAs on a genome-wide basis, and in a variety of tissues, we have developed numerous modifications and improvements to our original fluorescent in situ hybridization (FISH) protocol. To facilitate throughput and cost effectiveness, all steps, from probe generation to signal detection, are performed...

Multispectral Colour Constancy

Does extending the number of channels from the 3 RGB sensors of a colour camera to 6 or 9 using a multispectral camera enhance the performance of illumination-estimation algorithms? Experiments are conducted with a variety of colour constancy algorithms (Maloney-Wandell, Chromagenic, Greyworld, Max RGB, and a Maloney-Wandell extension) measuring their performance as a function of the number of ...

Multiplex fluorescent in situ hybridization in zebrafish embryos using tyramide signal amplification.

One of the strengths of the zebrafish is the ease with which in situ hybridization can be performed to determine spatial and temporal patterns of gene expression in whole embryos. Thus far, colorimetric detection methods are mainly used for these analyses. Here we describe a fluorescent in situ hybridization (FISH) protocol for whole-mount zebrafish embryos using tyramide signal amplification (...